Opened 7 years ago

Last modified 6 years ago

#615 assigned task

qRT-PCR: Primer manager

Reported by: Rene Ranzinger Owned by: sena
Priority: normal Milestone: Wish list
Component: qrtPCR management Version: GRITS 1.1.x
Severity: major Keywords:
Cc: sena

Description (last modified by Rene Ranzinger)

Implementation of a manager tool for the PCR primers that works before the current master gen list manager:

  • User enters primer design information
    • Primer sequence (forward and reverse)
    • Gen ID for which the primer is designed
  • After ordering the primer a PCR experiment is performed with known DNA
    • Plate layout
      • 1 Primer per row
      • replicates of 2
      • 5 different concentrations
      • Water as negative control
    • This data should be associated with the primer since it serves as Q/C data
      • it can be analysed as normal PCR experiment
      • in addition a curve needs to be generated for the different concentrations
      • R2 for the fit
  • After the experiment a primer can be transferred to the master gen list if he is working properly
    • the Q/C data should still be available
  • None successful primers should be stored as well so we can search for them later on
    • They also should have their Q/C data attached with it

Change History (3)

comment:1 Changed 7 years ago by Rene Ranzinger

Description: modified (diff)
Owner: set to sena
Status: newassigned

comment:2 Changed 7 years ago by Rene Ranzinger

Milestone: GRITS - 1.1 SR1GRITS - 1.1 SR2

comment:3 Changed 6 years ago by sena

Milestone: GRITS - 1.2Wish list
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